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by Andrew E. Nixon (Editor)
In this volume we will cover technologies and associated methodologies that allow identification, characterization and application of therapeutic peptides.
The section covering identification will cover areas such as display on the surface of filamentous or lytic phage, substrate phage display, ribosome display and the use of peptide library pools.
Often the output from library selection is not a peptide but the sequence of a peptide. We will therefore discuss approaches to produce peptide ligands both synthetically and through recombinant techniques. This section will also include a discussion of how peptides may be produced such that their serum residence time can be extended to allow weekly dosing such as fusions to other larger proteins or through modification with PEG.
The next section covers biophysical tools for characterization of peptide interactions with proteins. These include fluorescence polarization, equilibrium dialysis, radioligand binding assays, regular and array based SPR analysis.
The remaining section outlines methods for preparation and application of peptides as: imaging agents; targeting agents for radionuclei; receptor antagonists, and as tools to mediate cell penetration
Back Jacket
Therapeutic Peptides: Methods and Protocols features biological methods for the preparation of peptide phage display libraries using both filamentous and lytic phage. With contributions from renowned authors in the field, the book also explores selection and screening of the prepared peptide libraries for peptides with the desired function and the subsequent characterization of the identified peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and dependable, Therapeutic Peptides: Methods and Protocols is an ideal guide for researchers from all backgrounds seeking methods for the identification of therapeutic peptide candidates.
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