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by Chrissa Kioussi (Editor)
1. Culturing and Differentiating Mouse Embryonic Stem Cells
Axel P. Gross and Chrissa Kioussi
2. Neural Stem Cells Transplantation in an Animal Model of Traumatic Brain Injury
Dimitra Thomaidou
3. Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration
Ana Alastrue-Agudo, Slaven Erceg, Marta Cases-Villar, Viviana Bisbal-Velasco, Richard J. Griffeth, Francisco Javier Rodriguez-Jiménez, and Victoria Moreno-Manzano
4. Generation of Murine Xenograft Models of Brain Tumors from Primary Human Tissue for in vivo Analysis of the Brain Tumor Initiating Cell
Maleeha Qazi, Aneet Mann, Randy van Ommeren, Chitra Venugopal, Nicole McFarlane, Parvez Vora, and Sheila K. Singh
5. Growth of Bone Marrow and Skeletal Muscle Side Population Stem Cells in Suspension Culture
Christina A. Pacak and Douglas B. Cowan
6. Isolation, Culture and Immunostaining of Skeletal Muscle Fibers to Study Myogenic Progression in Satellite Cells
Louise A. Moyle and Peter S. Zammit
7. Human Neural Crest Stem Cells Derived from Human Pluripotent Stem Cells
Qiuyue Liu, Andrzej Swistowski, and Xianmin Zeng
8. Dental Pulp Stem Cells (DPSC) Isolation, Characterization and Differentiation
Federico Ferro, Renza Spelat, and Chelsea S. Baheney
9. Dental Pulp Stem Cells Isolation and Osteogenic Differentiation: A Good Promise for Tissue Engineering
Adriana Di Benedetto, Claudia Carbone, and Giorgio Mori
10. Efficient Hepatic Differentiation of Human Induced Pluripotent Stem Cells in A Three-Dimensional Microscale Culture
Ran-Ran Zhang, Takanori Takebe, Leina Miyazaki, Maho Takayama, Hiroyuki Koike, Masaki Kimura, Masahirop Enomura, Yun-Wen Zheng, Keisuke Sekine, and Hideki Taniguchi
11. The Generation and Maintenance of Rat Induced Pluripotent Stem Cells
Tomoyuki Yamaguchi, Sanae Hamanaka, and Hiromitsu Nakauchi
12. Protocol for Cutaneous Wound Healing Assay in a Murine Model
Gitali Ganguli-Indra
13. Adipose Derived Stem Cells: Methods for Isolation and Applications for Clinical Use
Brian Mailey, Ava Hosseini, Jennifer Baker, Adam Young, Zeni Alfonso, Kevin Hicok, Anne M. Wallace, and Steven Cohen
14. In vitro Detection of Residual Undifferentiated Cells in Retinal Pigment Epithelial Cells Derived from Human Induced Pluripotent Stem Cells
Takuya Kuroda, Satoshi Yasuda, and Yoji Sato
15. Whole Mount Immunohistochemistry to Study Spermatological Stem Cells and Spermatogenic Lineage in Mice, Monkeys and Humans
Kathrin Gassei, Hanna Valli, and Kyle E. Orwig
16. Differentiating the Stem Cell Pool of Human Hair Follicle Outer Root Sheath into Functional Melanocytes
Marie Schneider, Christina Dieckmann, Kathrin Rabe, Jan-Christoph Simon, and Vuk Savkovic
17. Pancreas Development ex vivo: Culturing Embryonic Pancreas Explants on Permeable Culture Inserts, with Firbonectin-Coated Glass Microwells, or Embedded in Three-Dimensional MatrigelTM
Hung Ping Shih and Maike Sander
18. Ultra-Rapid Manufacturing of Engineered Epicardial Substitute to Regenerate Cardiac Tissue Following Acute Ischemic Injury
Vahid Serpooshan and Pilar Ruiz-Lozano
Back Jacket
Stem Cells and Tissue Repair: Methods and Protocols presents in-depth methods for the three major approaches of rejuvenating an aging or sick body: latent regenerative capacity stimulated in a targeted way, replacement organs grown de novo and surgically implanted, and tissue surgically implanted and coaxed to integrate and restore problem areas. The first approach taps into the latent regenerative capacity of particular tissues, such as muscle, skin, fat, or bone marrow. The second approach induces and grows pluripotent stem cells, drives their differentiation along certain pathways such as germ layers, neural crest, liver, teeth or retina, and cultures organs such as pancreas and heart. While the third approach involves various engraftment techniques for neural tissue. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Stem Cells and Tissue Repair: Methods and Protocols provides state-of the-art methods descriptions and the references therein that will provide a suitable starting point for exploring the vast literature that has already developed for regenerative medicine.
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