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by James L. Hartley (Editor)
1. Why Proteins in Mammalian Cells?
James L. Hartley
2. Large Scale Transfection of Mammalian Cells
Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm
3. Selection of High Expressing Mammalian Cells by Surface Display of Reporters
Christine T. DeMaria
4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles
Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash
5. Transfection of Difficult-to-Transfect Primary Mammalian Cells
Oliver Gresch and Ludger Altrogge
6. Stable Protein Expression in Mammalian Cells Using Baculoviruses
Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch
7. Using Matrix Attachment Regions to Improve Recombinant Protein Production
Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod
8. Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells
Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald
9. Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells
Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang
10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells
Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens
11. Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker
Say Kong Ng
12. Tools for Co-Producing Multiple Proteins in Mammalian Cells
Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia
13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases
Paula Magnelli, Alicia Bielik, and Ellen Guthrie
14. Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA
Renate Kunert and Karola Vorauer-Uhl
15. Methods for Constructing Clones for Protein Expression in Mammalian Cells
Takefumi Sone and Fumio Imamoto
16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells
Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito
Back Jacket
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.
Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
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