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by Jennifer J. Kohler (Editor), Steven M. Patrie (Editor)
1. Introduction to Glycosylation and Mass Spectrometry
Steven M. Patrie, Michael J. Roth and Jennifer J. Kohler
Part I - Enrichment and Isolation Methods
2. Tandem Lectin Weak Affinity Chromatography (LWAC) for Glycoprotein Enrichment
Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller
3. CSC Technology - Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells
Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid 4. Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment Yawei Xu, Lijuan Zhang and Haojie Lu 5. Incorporation of Unnatural Sugars for the Identification of Glycoproteins Balyn W. Zaro, Howard C. Hang, Matthew R. Pratt 6. Characterization of Membrane-associated Glycoproteins using Lectin Affinity Chromatography and Mass Spectrometry Yashu Liu, Jintang He and David M. Lubman 7. Sialic Acid Capture-and-Release and LC-MSn Analysis of Glycopeptides Jonas Nilsson and Göran Larson Part II - Sample Preparation 8. In-Solution Digestion of Glycoproteins for Glycopeptide-Based Mass Analysis
9. Nano-HPLC-MS of Glycopeptides Obtained After Non-specific Proteolysis
Gerhild Zauner, Carolien A. M. Koeleman, André M. Deelder and Manfred Wuhrer
Part III - Separation Methods
10. Glycopeptide Enrichment for MALDI-TOF Mass Spectrometry Analysis by Hydrophilic Interaction Liquid Chromatography Solid Phase Extraction (HILIC SPE)
Pia H nnerup Jensen, Simon Mysling, Peter H jrup and Ole N rregaard Jensen 11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection Alina D. Zamfir , Corina Flangea, Alina Serb3, Ana-Maria Zagrean4, Andreas M. Rizzi5 and Eugen Sisu 12. Structural Separations by Ion Mobility-MS for Glycomics and Glycoproteomics Larissa S. Fenn and John A. McLean Part IV - Quantitation Methods 13. Quantitative Analysis of Glycoprotein Glycans Ron Orlando 14. Stable Isotope Labeling of N-glycosylated Peptides by Enzymatic Deglycosylation for Mass Spectrometry-based Glycoproteomics Hiroyuki Kaji and Toshiaki Isobe
Peng Zhao, Stephanie H. Stalnaker, Lance Wells
16. Glycan Profiling: Label-free Analysis of Glycoproteins
Yoshinao Wada
Part V - Computational Tools
17. Introduction to Informatics in Glycoprotein Analysis
Kiyoko F. Aoki-Kinoshita
18. Software Tools for Glycan Profiling
Chuan-Yih Yu, Anoop Mayampurath, Haixu Tang
Part VI - Case Studies in Mass Spectrometry of Glycoproteins
19. Quantitative Characterization of Glycoproteins in Neurodegenerative Disorders using iTRAQ
Min Shi, Hyejin Hwang and Jing Zhang
20. Quantitative Proteomic Analysis of N-linked Glycoproteins in Human Tear Fluid
Lei Zhou and Roger W. Beuerman
21. Elucidation of N-glycosites Within Human Plasma Glycoproteins for Cancer Biomarker Discovery
Penelope Drake, Birgit Schilling, Brad Gibson and Susan Fisher
22. Characterizing the Glycosylation State of Therapeutic Recombinant Glycoproteins
Nicole Samuels, David Kates, Jun Liu, Joanne Severs
11. Separation and Identification of Glycoforms by Capillary Electrophoresis with Electrospray Ionization Mass Spectrometric Detection
Alina D. Zamfir , Corina Flangea, Alina Serb3, Ana-Maria Zagrean4, Andreas M. Rizzi
Back Jacket
Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry.
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